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ATCC
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BioResource International Inc
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Cell Biologics Inc
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Dawley Inc
enterocytes from the proximal half of male sprague-dawley rat small intestine Enterocytes From The Proximal Half Of Male Sprague Dawley Rat Small Intestine, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/enterocytes from the proximal half of male sprague-dawley rat small intestine/product/Dawley Inc Average 90 stars, based on 1 article reviews
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Takeda
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Portland Press Ltd
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Merck KGaA
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FUJIFILM
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AnLab Ltd
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Janssen
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Instech Laboratories
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Schmid GmbH
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Image Search Results
Journal: Molecular Medicine Reports
Article Title: Atractylenolide III inhibits epithelial-mesenchymal transition in small intestine epithelial cells by activating the AMPK signaling pathway
doi: 10.3892/mmr.2022.12614
Figure Lengend Snippet: Effect of ATL-III on the activity of IEC-6 cells. (A) Chemical structure of ATL-III. (B) Effect of ATL-III on the activity of IEC-6 cells was detected using an MTT assay. ATL-III, atractylenolide III.
Article Snippet:
Techniques: Activity Assay, MTT Assay
Journal: Molecular Medicine Reports
Article Title: Atractylenolide III inhibits epithelial-mesenchymal transition in small intestine epithelial cells by activating the AMPK signaling pathway
doi: 10.3892/mmr.2022.12614
Figure Lengend Snippet: ATL-III inhibits the invasion and migration of IEC-6 cells induced by TGF-β1. (A) Cell morphology after TGF-β1 treatment was observed under a microscope. (B) Cell morphology after ATL-III treatment was observed under a microscope. Magnification, ×200. (C) Migration of IEC-6 cells was detected using a wound healing assay. (D) Cell invasion was detected using a Transwell assay. Histograms of cell (E) migration and (F) invasion rates. (G) Expression level of MMP9, as detected via western blotting. Magnification, ×100. ***P<0.001 vs. control group; ## P<0.01, ### P<0.001 vs. TGF-β1 group; n≥3. ATL-III, atractylenolide III.
Article Snippet:
Techniques: Migration, Microscopy, Wound Healing Assay, Transwell Assay, Expressing, Western Blot, Control
Journal: Molecular Medicine Reports
Article Title: Atractylenolide III inhibits epithelial-mesenchymal transition in small intestine epithelial cells by activating the AMPK signaling pathway
doi: 10.3892/mmr.2022.12614
Figure Lengend Snippet: ATL-III inhibits the EMT of IEC-6 cells induced by TGF-β1. (A) Expression levels of protein associated with EMT, as detected via western blotting. (B) Expression levels of N-cadherin and (C) E-cadherin were detected using an immunofluorescence assay. Magnification, ×200. ***P<0.001 vs. control group; ## P<0.01, ### P<0.001 vs. TGF-β1 group; n≥3. ATL-III, atractylenolide III; EMT, epithelial-mesenchymal transition; ZO-1, zonula occludens-1.
Article Snippet:
Techniques: Expressing, Western Blot, Immunofluorescence, Control
Journal: Molecular Medicine Reports
Article Title: Atractylenolide III inhibits epithelial-mesenchymal transition in small intestine epithelial cells by activating the AMPK signaling pathway
doi: 10.3892/mmr.2022.12614
Figure Lengend Snippet: ATL-III activates the AMPK signaling pathway in IEC-6 cells. (A) Expression levels of p-AMPK and AMPK in the groups treated with different concentrations of ATL-III were measured using western blotting. (B) Expression levels of p-AMPK and AMPK in the groups treated with TGF-β1 and different concentrations of ATL-III were measured using western blotting. (C) Chemical structure of compound C. (D) Expression levels of p-AMPK and AMPK in the groups treated with TGF-β1, ATL-III and compound C were detected using western blotting. *P<0.05, ***P<0.001 vs. control group; ### P<0.001 vs. TGF-β1 group; △△P<0.01 vs. TGF-β1 + ATL-III (20 µmol/l) group; n≥3. ATL-III, atractylenolide III; AMPK, AMP-activated protein kinase; p-, phosphorylated; t-, total.
Article Snippet:
Techniques: Expressing, Western Blot, Control
Journal: Molecular Medicine Reports
Article Title: Atractylenolide III inhibits epithelial-mesenchymal transition in small intestine epithelial cells by activating the AMPK signaling pathway
doi: 10.3892/mmr.2022.12614
Figure Lengend Snippet: ATL-III inhibits the invasion, migration and EMT process of IEC-6 cells induced by TGF-β1 by activating the AMPK signaling pathway. (A) Cell migration, as determined using a wound healing assay. (B) Cells invasion was detected using a Transwell assay. Magnification, ×100. Histograms of cell (C) migration and (D) invasion rates. (E) Expression levels of MMP9 and proteins associated with EMT, as detected via western blotting. (F) N-cadherin and (G) E-cadherin expression was detected using an immunofluorescence assay. Magnification, ×200. ***P<0.001 vs. control group; ### P<0.001 vs. TGF-β1 group; △P<0.05, △△P<0.01 vs. TGF-β1 + ATL-III (20 µmol/l) group; n≥3. ATL-III, atractylenolide III; EMT, epithelial-mesenchymal transition; AMPK, AMP-activated protein kinase; ZO-1, zonula occludens-1.
Article Snippet:
Techniques: Migration, Wound Healing Assay, Transwell Assay, Expressing, Western Blot, Immunofluorescence, Control